HGFR/c-MET Antibody AF276 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHGFR/c-MET Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF276
ApplicationsWestern Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Blockade of Receptor-ligand Interaction, Immunocytochemistry, CyTOF-ready
Conjugate/TagUnconjugated
ReactivityHuman
HostGoat
TargetHGFR/c-MET
IsotypeIgG
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human HGF R/c-MET, Glu25-Thr932, Accession # P08581
ConcentrationLYOPH
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
NCBI Full Gene NameMET proto-oncogene, receptor tyrosine kinase
NCBI Gene Aliasesc-Met, HGFR, AUTS9, DA11, RCCP2, DFNB97
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Knockout of c-Cbl/Cbl-b slows c-Met trafficking resulting in enhanced signaling in corneal epithelial cellsThe Journal of biological chemistryOctober 1, 2023Tarvestad-Laise K, Ceresa BP.Coverslips were incubated for 1 h at room temperature with primary antibodies against c-Met (catalog no.: AF276; R&D Systems) and LAMP1 (catalog no.: 909; Cell Signaling Technologies). After washing, coverslips were

(2) Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionPLOS PathogensAugust 23, 2023Quynh T. Phan, Norma V. Solis, Max V. Cravener, Marc Swidergall, Jianfeng Lin, Manning Y. Huang, Hong Liu, Shakti Singh, Ashraf S. Ibrahim, Massimiliano Mazzone, et al.incubated with recombinant c-Met (# MET-H5227, ACROBiosystems) followed by an anti human c-Met antibody (# AF276, R&D systems). The bands were visualized by enhanced chemiluminescence. In parallel 10μg of protein

(3) MET Receptor Tyrosine Kinase Inhibition Reduces Interferon-Gamma (IFN-γ)-Stimulated PD-L1 Expression through the STAT3 Pathway in Melanoma CellsCancersJune 29, 2023Song KY, Han YH, Roehrich H, Brown ME, Torres-Cabala C, Giubellino A.(5% dry milk and 0.1% Tween 20 in Tris-buffered saline) overnight at 4 °C. Immunoblotting with MET (AF276, 0.5 μg/mL, R&D systems, Minneapolis, MN, USA), Phospho-MET (AF2480, 0.5 μg/mL, Tyr1234/1235 or AF3950,

(4) Genetic Ablation of the MET Oncogene Defines a Crucial Role of the HGF/MET Axis in Cell-Autonomous Functions Driving Tumor DisseminationCancersMay 13, 2023Modica C, Cortese M, Bersani F, Lombardi AM, Napoli F, Righi L, Taulli R, Basilico C, Vigna E.ELISA plate was coated with 100 ng/mL of an antibody that recognizes the extracellular domain of MET (AF276—R&D Systems, Minneapolis, MN, USA). After saturation with 0.5% BSA in PBS, cell lysates obtained from

(5) Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasisCell reportsMarch 28, 2023Zuo Q, Yang Y, Lyu Y, Yang C, Chen C, Salman S, Huang TY, Wicks EE, Jackson W 3rd, Datan E, et al.

(6) Expression and Prognostic Evaluation of the Receptor Tyrosine Kinase MET in Canine Malignant MelanomaVeterinary sciencesMarch 26, 2023Koo K, Wuenschmann A, Rendahl A, Song KY, Forster C, Wolf-Ringwall A, Borgatti A, Giubellino A.Tween 20 in Tris-buffered saline) overnight at 4 °C. Immunoblotting with MET (R&D systems, Cat. No. AF276), Phospho-MET (Cell Signaling Technology, Danvers, MA, USA; Tyr1234/1235, Cat. No.3077), Phospho-MET

(7) Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionbioRxiv : the preprint server for biologyFebruary 23, 2023Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, et al.incubated with recombinant c-Met (# MET-H5227, ACROBiosystems) followed by an anti human c-Met antibody (# AF276, R&D systems). The bands were visualized by enhanced chemiluminescence. In parallel 10μg of protein

(8) The Impact of Nanobody Density on the Targeting Efficiency of PEGylated LiposomesInternational journal of molecular sciencesNovember 29, 2022Mesquita BS, Fens MHAM, Di Maggio A, Bosman EDC, Hennink WE, Heger M, Oliveira S.of the primary antibody diluted 1:30 in blocking buffer (goat anti-human HGFR/c-MET antibody, cat. # AF276, R&D Systems, Minneapolis, MN, USA) for 45 min at room temperature (RT). The cells were washed twice

(9) Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading CapacityNanomaterials (Basel)December 21, 2021Ashita Nair, Jiyoon Bu, Piper A. Rawding, Steven C. Do, Hangpeng Li, Seungpyo Hongantibody, SC-15363, Santa Cruz Biotechnology, San Jose, CA, USA) and C-Met (polyclonal anti-c-Met antibody, AF276, R&D Systems, Minneapolis, MN, USA). All the grids were rinsed and floated on 10 nm gold conjugated

(10) Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targetsNature cell biologyDecember 1, 2021Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, et al.(HPA012783) and anti-EGFR (06-847) from Sigma-Aldrich. Anti-GPC-1 (Invitrogen, PA5-28055), anti-MET (AF276) and anti-CD81 (clone 454720; MAB4615) from R&D Systems. Anti-AREG (6R1C2.4) from Bristol-Myers Squibb

(11) The EMT activator ZEB1 accelerates endosomal trafficking to establish a polarity axis in lung adenocarcinoma cellsNature communicationsNovember 3, 2021Banerjee P, Xiao GY, Tan X, Zheng VJ, Shi L, Rabassedas MNB, Guo HF, Liu X, Yu J, Diao L, et al.dilution for immunofluorescence) from Cell Signaling Technologies; and primary antibody against MET (#AF276, 1:100 dilution for immunofluorescence) and recombinant human HGF protein (#294-HG-005/CF) from R&D Systems

(12) Autocrine signaling by receptor tyrosine kinases in urothelial carcinoma of the bladderPLOS ONEJuly 22, 2021Young H. Lee et al.from Cell Signaling Technology (Danvers, Massachusetts USA). Antibodies against Met (cat. # BAF358, AF276), RYK (cat. #AF4907), CSF1 (cat. #AF216), CSF1R (cat. #MAB3291) and GAS6 (cat. # AF885) were obtained

(13) RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesiseLifeJune 7, 2021Nászai M, Bellec K, Yu Y, Román-Fernández A, Sandilands E, Johansson J, Campbell AD, Norman JC, Sansom OJ, Bryant DM, et al.555651) for α5β1, anti-CD71 (BDPharmingen, Cat# 555534) for the TfnR, anti-HGFR (R&D Systems, Cat# AF276), and anti-EGFR1 (BDPharmingen, Cat# 555996). Statistical analysis GraphPad Prism 8 software was used

(14) Single nuclei RNA-seq of mouse placental labyrinth developmenteLifeNovember 3, 2020Bryan Marsh, Robert BlellochPolyclonal antibody,Abcam,ab51745,IF (1:50) Antibody,Anti-Met Polyclonal antibody,R and D Systems,AF276,IF (1:100) Antibody,Anti-Egfr Polyclonal antibody,R and D Systems,AF1280,IF (1:100) Antibody,Anti-Gata1

(15) MET-dependent solid tumours — molecular diagnosis and targeted therapyNature Reviews Clinical OncologySeptember 1, 2020Robin Guo, Jia Luo, Jason Chang, Natasha Rekhtman, Maria Arcila, Alexander Drilon[e.g. SP44 (Ventana), cMET (R&D systems), MET4 (Dako)], polyclonal antibodies (e.g. polyclonal MET AF276 (R&D systems) and antibodies to phosphorylated MET [pMET Y1349 (Epitomics)]173–177. Of these, SP44,

(16) The Role of Receptor Tyrosine Kinases in Lassa Virus Cell EntryVirusesAugust 6, 2020Fedeli C, Moreno H, Kunz S.previously [69]. Purified polyclonal goat IgG anti human Axl (#AF154) and anti-human HGFR (for IFA, #AF276) were purchased from R&D Systems (Minneapolis, MN, USA). Monoclonal 113 anti-LCMV NP antibody has been

(17) FCHSD2 controls oncogenic ERK1/2 signaling outcome by regulating endocytic traffickingPLoS biologyJuly 1, 2020Xiao GY, Schmid SL.with antibodies against the following proteins: p-EGFR Y1068 (#3777S, 1:200; Cell Signaling), MET (#AF276, 1:100; R&D Systems, Minneapolis, MN), EEA1 (#610457, 1:100; BD Biosciences, San Jose, CA), Rab11 (#5589S,

(18) Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand ActivationInternational Journal of Molecular SciencesApril 17, 2020Marie-Lena I.E. Harwardt et al.temperature for two hours. 2 µg/mL primary goat anti-human MET IgG (R&D Systems, Minneapolis, MN USA, AF276) and 4µg/mL mouse anti-EGFR IgG (Santa Cruz Biotechnology, Dallas, TX, USA, sc-120) were used. Samples

(19) Receptor-based mechanism of relative sensing and cell memory in mammalian signaling networkseLifeJanuary 21, 2020Lyashenko E, Niepel M, Dixit PD, Lim SK, Sorger PK, Vitkup D.(Cell Signaling Technologies, 4060, 1:400), FoxO3 (Cell Signaling Technologies, 2497, 1:200), anti-Met (R and D Systems, AF276, 1:150), or anti-EGFR (Thermo Fisher Scientific, MA513319, 1:100) over night at 4˚C. Cells were washed

(20) Arf6 regulates RhoB subcellular localization to control cancer cell invasionJournal of Cell BiologyNovember 4, 2019Kossay Zaoui, Charles V. Rajadurai, Stéphanie Duhamel, Morag Parkbefore the addition of antibodies. The following antibodies were used for immunofluorescence: Met (AF276; R&D Systems); RhoB (sc-8048) and RhoB (sc-180; Santa Cruz Biotechnology; Fig. S1 D); Arf6 (AB77581;

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HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

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